CpG DNA-mediated immune response in pulmonary endothelial cells
Identifieur interne : 002034 ( Main/Exploration ); précédent : 002033; suivant : 002035CpG DNA-mediated immune response in pulmonary endothelial cells
Auteurs : JIANG LI [États-Unis] ; ZHENG MA [États-Unis] ; Zhi-Lue Tang [États-Unis] ; Troy Stevens [États-Unis] ; Bruce Pitt [États-Unis] ; SONG LI [États-Unis]Source :
- American journal of physiology. Lung cellular and molecular physiology [ 1040-0605 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Although the CpG DNA immune response mediated by Toll-like receptor 9 (TLR9) has been extensively studied in a number of immune cells, the response to CpG DNA in endothelial cells (EC) is not well understood. In this study, we show that both mouse and rat lung EC display constitutive expression of TLR9 mRNA. Exposure to CpG DNA induced a potent proinflammatory response as manifested by an increased expression of IL-8 and ICAM-1 in mouse pulmonary EC. The proinflammatary response was sensitive to chloroquine, consistent with a role of endosomal contribution. A role for p38 MAPK and NF-KB pathway was apparent as the response was sensitive to inhibitors of p38 MAPK and NF-KB but was not affected by inhibitors of ERK1/2. A synergistic effect of CpG DNA and LPS on the inflammatory response is consistent with multiple TLR interaction in EC. This study suggests a possible role for CpG DNA-mediated EC immune response in the host defense system. It also has important implications in plasmid DNA-mediated pulmonary endothelium gene transfer.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000046
- to stream PascalFrancis, to step Curation: 000020
- to stream PascalFrancis, to step Checkpoint: 000048
- to stream Main, to step Merge: 002050
- to stream Main, to step Curation: 002034
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">CpG DNA-mediated immune response in pulmonary endothelial cells</title><author><name sortKey="Jiang Li" sort="Jiang Li" uniqKey="Jiang Li" last="Jiang Li">JIANG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Zheng Ma" sort="Zheng Ma" uniqKey="Zheng Ma" last="Zheng Ma">ZHENG MA</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Tang, Zhi Lue" sort="Tang, Zhi Lue" uniqKey="Tang Z" first="Zhi-Lue" last="Tang">Zhi-Lue Tang</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Environmental and Occupational Health, Graduate School of Public Health University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Stevens, Troy" sort="Stevens, Troy" uniqKey="Stevens T" first="Troy" last="Stevens">Troy Stevens</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Pharmacology, Center for Lung Biology, University of South Alabama, College of Medicine</s1><s2>Mobile, Alabama 36688</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mobile, Alabama 36688</wicri:noRegion></affiliation></author><author><name sortKey="Pitt, Bruce" sort="Pitt, Bruce" uniqKey="Pitt B" first="Bruce" last="Pitt">Bruce Pitt</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Environmental and Occupational Health, Graduate School of Public Health University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Song Li" sort="Song Li" uniqKey="Song Li" last="Song Li">SONG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0232512</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 05-0232512 INIST</idno><idno type="RBID">Pascal:05-0232512</idno><idno type="wicri:Area/PascalFrancis/Corpus">000046</idno><idno type="wicri:Area/PascalFrancis/Curation">000020</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000048</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000048</idno><idno type="wicri:doubleKey">1040-0605:2004:Jiang Li:cpg:dna:mediated</idno><idno type="wicri:Area/Main/Merge">002050</idno><idno type="wicri:Area/Main/Curation">002034</idno><idno type="wicri:Area/Main/Exploration">002034</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">CpG DNA-mediated immune response in pulmonary endothelial cells</title><author><name sortKey="Jiang Li" sort="Jiang Li" uniqKey="Jiang Li" last="Jiang Li">JIANG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Zheng Ma" sort="Zheng Ma" uniqKey="Zheng Ma" last="Zheng Ma">ZHENG MA</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Tang, Zhi Lue" sort="Tang, Zhi Lue" uniqKey="Tang Z" first="Zhi-Lue" last="Tang">Zhi-Lue Tang</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Environmental and Occupational Health, Graduate School of Public Health University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Stevens, Troy" sort="Stevens, Troy" uniqKey="Stevens T" first="Troy" last="Stevens">Troy Stevens</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Pharmacology, Center for Lung Biology, University of South Alabama, College of Medicine</s1><s2>Mobile, Alabama 36688</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Mobile, Alabama 36688</wicri:noRegion></affiliation></author><author><name sortKey="Pitt, Bruce" sort="Pitt, Bruce" uniqKey="Pitt B" first="Bruce" last="Pitt">Bruce Pitt</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Environmental and Occupational Health, Graduate School of Public Health University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Song Li" sort="Song Li" uniqKey="Song Li" last="Song Li">SONG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh</s1><s2>Pittsburgh, Pennsylvania 15261</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author></analytic><series><title level="j" type="main">American journal of physiology. Lung cellular and molecular physiology</title><title level="j" type="abbreviated">Am. j. physiol., Lung cell. mol. physiol.</title><idno type="ISSN">1040-0605</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">American journal of physiology. Lung cellular and molecular physiology</title><title level="j" type="abbreviated">Am. j. physiol., Lung cell. mol. physiol.</title><idno type="ISSN">1040-0605</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA</term><term>Endothelial cell</term><term>Immune response</term><term>Lung</term><term>Mammalia</term><term>Respiratory system</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>DNA</term><term>Réponse immune</term><term>Poumon</term><term>Cellule endothéliale</term><term>Mammalia</term><term>Appareil respiratoire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although the CpG DNA immune response mediated by Toll-like receptor 9 (TLR9) has been extensively studied in a number of immune cells, the response to CpG DNA in endothelial cells (EC) is not well understood. In this study, we show that both mouse and rat lung EC display constitutive expression of TLR9 mRNA. Exposure to CpG DNA induced a potent proinflammatory response as manifested by an increased expression of IL-8 and ICAM-1 in mouse pulmonary EC. The proinflammatary response was sensitive to chloroquine, consistent with a role of endosomal contribution. A role for p38 MAPK and NF-KB pathway was apparent as the response was sensitive to inhibitors of p38 MAPK and NF-KB but was not affected by inhibitors of ERK1/2. A synergistic effect of CpG DNA and LPS on the inflammatory response is consistent with multiple TLR interaction in EC. This study suggests a possible role for CpG DNA-mediated EC immune response in the host defense system. It also has important implications in plasmid DNA-mediated pulmonary endothelium gene transfer.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Jiang Li" sort="Jiang Li" uniqKey="Jiang Li" last="Jiang Li">JIANG LI</name></region><name sortKey="Pitt, Bruce" sort="Pitt, Bruce" uniqKey="Pitt B" first="Bruce" last="Pitt">Bruce Pitt</name><name sortKey="Song Li" sort="Song Li" uniqKey="Song Li" last="Song Li">SONG LI</name><name sortKey="Stevens, Troy" sort="Stevens, Troy" uniqKey="Stevens T" first="Troy" last="Stevens">Troy Stevens</name><name sortKey="Tang, Zhi Lue" sort="Tang, Zhi Lue" uniqKey="Tang Z" first="Zhi-Lue" last="Tang">Zhi-Lue Tang</name><name sortKey="Zheng Ma" sort="Zheng Ma" uniqKey="Zheng Ma" last="Zheng Ma">ZHENG MA</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002034 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002034 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:05-0232512
|texte= CpG DNA-mediated immune response in pulmonary endothelial cells
}}
| This area was generated with Dilib version V0.6.33. |  |